Human Tau ELISA Kit
$699.00
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 16 pg/ml
- Range: 40.63 pg/ml – 2600 pg/ml
- Sample type: Cell culture extracts, Cell culture media, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 5.5% Inter-assay Sample n Mean SD CV% Extract 3 2.7% -
Sample type
Serum, Cell culture extracts, Tissue Extracts, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16 pg/ml -
Range
40.63 pg/ml – 2600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 79 76% – 84% Cell culture extracts 97 98% – 101% Tissue Extracts 108 101% – 111% Cell culture media 86 84% – 91% Hep Plasma 75 70% – 82% EDTA Plasma 78 75% – 83% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Tau ELISA kit (ab273617) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Tau protein in serum, plasma, cerebrospinal fluid, cell culture supernatant and cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tau with 16 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Tau proteins constitute nine isoforms from a single transcript from the MAPT gene that range from 33-81 kDa. Tau proteins are expressed mainly in the neurons of the central nervous systems. They promote microtubule assembly and stability and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus of Tau binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that Tau functions as a linker protein between both. Hyperphosphorylation of Tau proteins can result in destabilization of microtubule organization, Tau aggregation, and tangle formation. Defective Tau proteins may play a role in diseases of the nervous systems, including Alzheimer disease, Pick disease of the brain, Progressive supranuclear palsy 1 and Parkinson-dementia syndrome. Based on the immunogen design of this ELISA antibody pair, this kit should detect all nine human Tau isoforms and it should have equal affinity towards human, mouse and rat Tau proteins.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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