Human Amyloid Precursor Protein ELISA Kit
$685.00
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 14.9 pg/ml
- Range: 93.75 pg/ml – 6000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 3 6.2% Inter-assay Sample n Mean SD CV% Serum 5 5.1% -
Sample type
Cell culture supernatant, Urine, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
14.9 pg/ml -
Range
93.75 pg/ml – 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 112 107% – 117% Urine 93 86% – 100% Serum 112 111% – 114% Cell culture extracts 93 90% – 97% Tissue Extracts 112 107% – 118% Hep Plasma 106 104% – 109% EDTA Plasma 109 93% – 123% Cit plasma 91 85% – 105% Cerebral Spinal Fluid 101 92% – 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Amyloid Precursor Protein ELISA Kit (ab216944) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Amyloid Precursor Protein protein in cell culture extracts, cell culture supernatant, cerebral spinal fluid, cit plasma, edta plasma, hep plasma, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Amyloid Precursor Protein with 14.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
– Single-wash protocol reduces assay time to 90 minutes or less
– High sensitivity, specificity and reproducibility from superior antibodies
– Fully validated in biological samples
– 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Amyloid Precursor Protein (GeneCards: APP) is a multifunctional transmembrane protein that consists of a 682 amino acid (aa) long extracellular domain, a 24 aa long transmembrane segment, and a 47 aa long cytoplasmic domain. Alternative splicing generates multiple isoforms including the most prevalent APP695, APP751, and APP770. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Amyloid Precursor Protein functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Amyloid Precursor Protein is involved in cell mobility and transcription regulation through protein-protein interactions.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
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