Cortisol ELISA Kit
$637.00
Key features and details
- Sensitivity: 2.44 ng/ml
- Range: 10 ng/ml – 500 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
-
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 60 <=9% Inter-assay Sample n Mean SD CV% Overall 30 <=9.8% -
Sample type
Urine, Serum, Hep Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
= 2.44 ng/ml -
Range
10 ng/ml – 500 ng/ml -
Recovery
103.56 %
-
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in serum and plasma.
A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where any Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.
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